However, they can be freed from contaminating substances by steam distillation at a lower temperature, at which they are stable. © 1997-2020 LUMITOS AG, All rights reserved,, Your browser is not current. In addition to the degradation caused by the seed, the exponential decreased of COD concentration observed after 3 hours of biodegradation may also be due to the addition of 300 mL dilution water which contains the nutrients needed by the microorganisms to survive.

Figure 3 shows that after 15 hours of biodegradation (1st COD monitoring), COD concentrations of 2000 ppm, 4000 ppm, 6000 ppm, 8000 ppm, and 10000 ppm KHP samples were reduced by 34.76%, 36.28%, 35.74%, 34.10% and 38.40% respectively.

The degraded benzene sample was then subjected for GC-MS analysis.

Potassium hydrogen phthalate is irritant and should be handled with care. Figure 4: The graph obtained from plotting COD concentrations of benzene and KHP controls (unseeded and aerated) against biodegradation time.

The 1000 ppm benzene sample was 99.79% degraded from an initial COD concentration of 1112.545 ppm to 2.352 ppm. The biodegradability of KHP samples (2000 ppm, 4000 ppm, 6000 ppm, 8000 ppm and 10000 ppm) using BOD seed inoculum was studied for 207 hours (9 days). As shown in Table 1, DO concentration of benzene increased from 3.8 mg/L to 6.1 mg/L while DO of KHP increased from 2.4 mg/L to 8.6 mg/L. The sample was stoppered immediately and was inverted two to three times gently. Monitoring was conducted through COD test. Biodegradation uses pure microbial structure as well as mixed microbial cultures. [1] It is also used as a primary standard for calibrating pH meters because, besides the properties just mentioned, its pH in solution is very stable. Aside from the biodegradability of the samples, a negative, blank, and sample control were used.

The pH of a 0.05 mol kg−1 aqueous solution of KHP at various temperatures is given by the following table:[2]. It is also used as an organic solvent for various experiments and as a raw material for the manufacture of plastic, paper, detergents, and pesticides [2]. h��W_O�8�G�#�@��q��V��B�J�E��*L���I��b���݌��ˊ;�� ���3�_��r�?�~�oI}"� �QT�����Ih(`N#!��J�,h�y�� ;��AHA�8ҥ ��e����٨��/`�����&c㪾�jvC��Q�Xr�����e��K!�l��۴��uV��g��]�����scT�U{td�ߘ�C)��}����/'�d��d���k�M�vd. For the state in East Asia which is abbreviated as КНР in Russian, see, InChI=1S/C8H6O4.K/c9-7(10)5-3-1-2-4-6(5)8(11)12;/h1-4H,(H,9,10)(H,11,12);/q;+1/p-1, InChI=1/C8H6O4.K/c9-7(10)5-3-1-2-4-6(5)8(11)12;/h1-4H,(H,9,10)(H,11,12);/q;+1/p-1, Except where otherwise noted, data are given for materials in their, Smalley, I.J.,Lill,G.O.,Bentley,S.P.,Wood,D.R. An example of a standardized inoculum is the BOD5 Seed Inoculum which is known as the standard inoculum use in BOD5 test. This only implies the effectivity of the BOD5 Seed Inoculum in the biodegradation of aqueous organic waste. Material Safety Data Sheet Potassium hydrogen phthalate ACC# 19425 Section 1 - Chemical Product and Company Identification Biodegradation of higher concentration of KHP. In biodegradability testing, important factors that cannot be easily standardized should be given enough attention for it has a great influence in the biodegradation results; which includes the inoculum, the source of the microorganisms for the test, and its state of acclimatization and adaptation. The increased DO indicate that the samples were degraded since increased DO means that organic substances present in the sample have decreased which allows more oxygen to be dissolved in the solution. Phthalic acid Phthalic anhydride : Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). At final COD monitoring, initial COD concentration of benzene and KHP control was reduced by 8.09% and 3.29%, respectively. SODIUM HYDROXIDE Page 4 of 6 For more information, please contact: New Jersey Department of Health Right to Know PO Box 368 Trenton, NJ 08625-0368ma Phone: 609-984-2202 1977. Use water from the sink nearest the bench to douse the flames. The neutralization of the sample was obtained by adjustment of pH with the use of Sulfuric Acid and Sodium Hydroxide (LobaChemie Pvt. Skin: Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. 2���H�00�����2 �v"? The BOD5 Seed Inoculum had also been used to treat aqueous organic waste sample to test the effectivity of its biodegradation for aqueous organic waste. Same instance was observed for the waste sample in which its DO concertation increased from 1.1 mg/L to 1.7 mg/L. I need to calculate the mass of KHP in unknown... and the percent of KHP in the unknown based on this information.

The ‘standard’ inoculum could be formed by mixing specific microbial strains conserved in microbial collections with fine knowledge of its relevant biochemical properties. Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members. Berina LIM, Ricohermoso SDA, Tejada VAC, Bautista CCJ, Abigail CAP (2018) Biodegradability Study of Potassium Hydrogen Phthalate and Benzene Using BOD5 Seed as Inoculum.

Ltd, India) bought from Belman Laboratories (Quezon City, Metro Manila) was used as the primary sample for the biodegradation. On the other hand, higher concentrations of KHP sample were not degraded. With an accout for you can always see everything at a glance – and you can configure your own website and individual newsletter. In DO analysis, organic substances present in the sample tend to snatched dissolved oxygen in the sample.

1312 0 obj <>/Filter/FlateDecode/ID[<0AC18EC44BB8A54985135E29BB20D37C><64F346E88DE4AF43803CCB98E7243278>]/Index[1297 30]/Info 1296 0 R/Length 83/Prev 116239/Root 1298 0 R/Size 1327/Type/XRef/W[1 2 1]>>stream 2020 - Open Access Publisher. In this lab students will determine the concentration of a sodium hydroxide (NaOH) solution using the primary standard potassium hydrogen phthalate (KHP).Once standardized, students will use the NaOH solution to determine the concentration of an acetic acid (CH 3 COOH) solution, in this case white table vinegar labeled as 5% by mass. The samples were tested for Dissolved Oxygen (DO) and Chemical Oxygen Demand (COD) Test for initial analysis before it was prepared for the biodegradation process.


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