Low cost. Deb and Imchen (2010) reported an, ., 1995). In the recent past several attempts, have been made to look for suitable substrata that, could possibly replace agar in culture medium because, of doubts about its inertness and nontoxic nature, fear, of over-exploration of its sources and above all, the, high cost of tissue culture grade agar (Babbar and. One should always try that the price of tissue culture, raised plants must be competitive with those obtained, from conventional propagation. etc., medicinal and aromatic plants and trees (Bajaj, a very important technology which can be used for, production of large scale quality planting materials of, selected novel high yielding varieties to boost, production. Aggarwal S Sharma S and Pathak P 2006 On the utility of coir as, Ahloowalia BS 2000 Stability of micropropagated plants and, mini-tubers of potato In : Methods and Markers for, Quality Assurance in Micropropagation Cassells, Ahloowalia BS 2004 Integration of technology from lab to land, In : Low cost option for tissue culture technology in, developing countries Proceedings of a Technical, Ahloowalia BS 1999b Stability of micropropagated plants and, mini-tubers of potato In: Methods and Markers for, Quality Assurance in Micropropagation Eds Cassells, Ahloowalia BS and Prakash J 2004 Physical components in tissue, culture technology In : Low cost option for tissue, culture technology in developing countries Proceedings, Ahloowalia BS and Prakash J 2004 Incorporation of low cost, options In : Low cost option for tissue culture, technology in developing countries Proceedings of a, energy and labour In : Low cost option for tissue culture, Plant tissue culture In : Low cost option for tissue, Aitken-Christie J 1991 Automation In: Micropropagation Eds, Debergh PC Zimmerman RH Dordrecht: Kuluwer, Ammirato P 1983a Embryogenesis In: Evans DA Sharp WR, Anonymous 2001 Solatube the miracle skylight http://, Anonymous 2002 Indian tissue culture industry Part-I Dalal, Consultants and Engineers Ltd Floriculture Today June, Bateson JM Grout BWW and Lane S 1987 The influence of, container dimensions on the multiplication rate of, regenerating plant cell cultures In : Plant, Micropropagation in Horticultural Industries, Preparation Hardening and Acclimatization Processes, Ducate G Jacob M and Simeon a Eds Belgian Plant, Tissue Culture Group Symposium Florizel 87, Scientific Journal of Can Tho University 7 : 222-30 in, Bajaj YPS 1986 Biotechnology of tree improvement for rapid, propagation and biomass energy production In: Bajaj, YPS ed Biotechnology in Agriculture and Forestry vol, I Trees I Springer Berling Heidelberg New Y, Bajaj YPS Furmanova M and Olszowska O 1988 Biotechnology, of the micropropagation of medicinal and aromatic, plants In: Bajaj YPS ed Biotechnology in Agriculture, growth under a light source with and without blue, Bhattacharya P Dey S and Bhattacharya BC 1994, Current Microbiology and Applied Sciences, sugars on growth and development of coconut, Boxus Ph 1978 The production of fruit and vegetable plants by, In: Propagation of Higher Plants through Tissue, Bula RJ Morrow RC Tibbitts TW and Barta DJ 1991, WR Bajaj YPS eds Handbook of plant cell culture vol 5, Debergh PC and Zimmerman RH 1991 Micro-propagation, publishers Dordrecht The Netherlands 7 : 1-93, Pruski K 2002 Priming of Tissue culture propagules, In: Low cost options for tissue culture technology in, developing countries Proceedings of a technical meeting, techniques in food and agriculture August 26-30 2002, In: “Tissue culture as a plant production system for, horticultural crops Zimmerman RH Grierbach RJ, Dutta I Bhadra J Ghosh P Saha B and Datta S, Emmerich JC Morrow RC Clavette TJ and Sirios LJ 2004 Plant, Research Unit lighting system development SAE, Technical Paper Series Paper No 2004-01-2454, Gangopadhyay G Bandyopadhyay T Basu Gangopadhyay S, Gangopadhyay G Das S Mitra SK Poddar R Modak BK and, Ganapath TR Mohan JSS Suprasanna P Bapat V. Gebre E and Sathyanarayana BN 2001 Tapioca : cheaper alternative to agar for direct in vitro, regeneration and microtuber production from nodal, George EF and Sherrington PD 1984 Commercial tissue culture, laboratories In: George EF Sherrington PD eds Plant, propagation by tissue culture Handbook and directory, of commercial laboratories Easter Press Great Britain, substitution on micro shoot production of two potato, Gitonga NM Ombori O Murithi KSD and Ngugi M 2010, Sager JC 2001 Salad crop production under different, wavelengths of red light emitting diodes SAE Technical, development and plant regeneration in twenty two, indigenous and exotic orchid species and hybrids M Sc, Hoque MI Roy AR Sarker RH and Haque MM 1994, microbial media BSc Dissertation University of, Malawi Bunda College of Agriculture Lilongwe, Kieffer M Simkins N Fuller MP and Jellings AJ 2001, Kim HH Goins GD Wheeler RM and Sager JC 2004, Komol N 1998 Study on the development of low cost medium, for large scale propagation of some orchids MSc Thesis, Kozai T and Kubota C 1997 Greenhouse technology for saving, production in closed ecosystems 139-152 Kluwer, Kwame O Gitonga N Mburugu MM Omwoyo O and Michael N, Lim S Seon JH Pack KY Son SH and Han BH 1998, MacLennan DA et al 1995 Efficient full-spectrum long-lived, non-toxic microwave lamp for plant growth Proc of, International lighting in controlled environments, an efficient LED intracanopy lighting design for, minimizing equivalent system mass in an advanced, Massa GD Emmerich JC Morrow RC and Mitchell CA 2005b, Development of a reconfigurable LED plant-growth, lighting system for equivalent system mass reduction, Cassava Starch as Alternative to Agar for Gelling Tissue, cassava an ancient crop for modern times held at Donald, Danforth Plant Science Centre St Louis Missouri pp, McCulloch SM Briggs BA and Spethmann W 1994, Mehrotra S Goel MK Kukreja AK and Mishra BN 2007, Mohan R Chui1 EA Biasi LA and Soccol RC 2005, Moraes-Cerdeira R M Krans J V McChesney J D Pereira, Morrow RC Duffie NA Tibbitts TW Bula RJ Barta DJ Ming, DW Wheeler RM and Porterfield DM 1995 Plant, unit SAE Technical Paper Series Paper No 951624. Micronutrient requirements for maximization of, sugarcane yield has been standardized (Savangikar, The commercial production of ornamental plants is, growing worldwide. Deb and Arenmongla (2014) screened some low cost, substrata as alternative to agar for in vitro, Among the three alternative substrata incorporated, in the regeneration medium, regenerative response on, ‘foam disk’ as substratum was competitive with agar, Experiment indicated that 100 percent sucrose, supplementation during proliferation reduces the, chemical cost by 15.12 percent without causing any, significant difference among the number of multiple, shoots. conservation of important medicinal plants resources. Costly, aluminium foil is used for wrapping the instruments, before sterilization. T, ever-increasing demand for quality transplants, the, scaling-up of photoautotrophic micropropagation, systems, for commercialization, has become, advancement in the development and utilization of large, culture vessels for photoautotrophic micropropagation, with special emphasis on the feasibility of the system, for the commercial-scale propagation (Zobayed, A cost effective micropropagation protocol has been, developed successfully using both conventional and, unconventional low cost alternatives for the. Plant tissue culture is a very important technology which is used for production of large scale quality (culture containers, autoclave, laminar flow, instruments used for micropropagation, pH meter etc). multiplication of orchids (Hossain, 1995; Pervin, 1997; dust, sand, refuse tea, sago, cassava flour, rice polish, compost, kithul flour etc. Artificial lighting of cultures in the growth rooms is, one of the most expensive methods in tissue culture, technology. To develop new and novel ornamental varieties through traditional mutation; in vitro mutagenesis, and classical breeding etc. In spite of these merits, the, micropropagation technique has certain demerits also, Plant tissue culture is a very important technology which is used for production of large scale quality, planting materials of selected novel high yielding varieties to boost production. Micropropagation, popularly known for large-, scale clonal propagation, is the first major and widely. micropropagation (Debergh and Mane, 1981; Donnan. It offers many unique advantages over conventional, propagation methods such as rapid multiplication of, valuable genotypes, expeditious release of improved, varieties, production of disease-free quality planting.

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